Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99816

AMBRA1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

AMBRA1 rabbit pAb from ELK Biotechnology Read more ›

AMBRA1 rabbit pAb
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AMBRA1 rabbit pAb

Product Description

AMBRA1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AMBRA1 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.,similarity:Contains 3 WD repeats.,subunit:Interacts with BECN1. Probably forms a complex with BECN1 and PIK3C3.,

Additional antibody information

Specificity AMBRA1 Polyclonal Antibody detects endogenous levels of AMBRA1
Validation evidence captions Western Blot analysis of 293T 3T3 cells using AMBRA1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: AMBRA1
Collaborator ELK Biotechnology
Partner model ES8480
Target AMBRA1
Research areas>>Mitophagy - animal, >>Autophagy - animal, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Spinocerebellar ataxia, >>Pathways of neurodegeneration - multiple diseases
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic Peptide of AMBRA1
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
AMBRA1
Also known as
autophagy/beclin-1 regulator 1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
Synthetic Peptide of AMBRA1
Observed band
140kD
Cellular localization
Endoplasmic reticulum . Cytoplasm, cytoskeleton . Cytoplasmic vesicle, autophagosome . Mitochondrion . Cytoplasm, cytosol . Nucleus . Cell junction, focal adhesion . Localizes to the cytoskeleton in absence of autophagy induction (PubMed:20921139). Upon autophagy induction, AMBRA1 relocalizes to the endoplasmic reticulum to enable autophagosome nucleation (PubMed:20921139). Partially localizes at mitochondria in normal conditions (PubMed:21358617). Localizes also to discrete punctae along the ciliary axoneme (By similarity). .
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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