PI 3 Kinase Class 3 rabbit pAb from ELK Biotechnology Read more ›
PI 3 Kinase Class 3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying PI 3 Kinase Class 3 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat, Predicted, Cow, Pig. Specifications and supporting documents below should be reviewed when planning the experiment.
catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.,cofactor:Manganese.,function:Catalytic subunit of the PI3K complex. Involved in the transport of lysosomal enzyme precursors to lysosomes.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 PI3K/PI4K domain.,subunit:Probably forms a complex with AMBRA1 and BECN1 (By similarity). Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4.,tissue specificity:Ubiquitously expressed, with a highest expression in skeletal muscle.,
| Specificity | This antibody detects endogenous levels of PI 3 Kinase Class 3 Polyclonal Antibody at Human;Mouse;Rat;Predicted:Cow:PIG |
|---|---|
| Validation evidence captions | Western Blot analysis of 293T 3T3 cells using PI 3 Kinase Class 3 Polyclonal Antibody diluted at 1:1500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES8477 |
| Target | PI 3 Kinase Class 3 |
| Research areas | >>Inositol phosphate metabolism, >>Metabolic pathways, >>Phosphatidylinositol signaling system, >>Autophagy - other, >>Autophagy - animal, >>Phagosome, >>Apelin signaling pathway, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Spinocerebellar ataxia, >>Pathways of neurodegeneration - multiple diseases, >>Shigellosis, >>Salmonella infection, >>Tuberculosis, >>Kaposi sarcoma-associated herpesvirus infection |
| Reactivity | Human,Mouse,Rat,Predicted,Cow,Pig |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide of PI 3 Kinase Class 3 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed, with a highest expression in skeletal muscle. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Predicted:Cow:PIG | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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