NRF-1 rabbit pAb from ELK Biotechnology Read more ›
NRF-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NRF-1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Bovine. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014],
| Specificity | NRF-1 Polyclonal Antibody detects endogenous levels of NRF-1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using NRF-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western blot analysis of NRF1 Antibody. The lane on the right is blocked with the NRF1 peptide. | Western blot analysis of the lysates from HeLa cells using NRF1 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6416 |
| Target | NRF-1 |
| Research areas | >>Apelin signaling pathway, >>Huntington disease |
| Reactivity | Human,Mouse,Rat,Bovine |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NRF1. AA range:221-270 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed with strongest expression in skeletal muscle. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Bovine |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Bovine | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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