Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97405

MRP-L11 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MRP-L11 rabbit pAb from ELK Biotechnology Read more ›

MRP-L11 rabbit pAb
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MRP-L11 rabbit pAb

Product Description

MRP-L11 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MRP-L11 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This nuclear gene encodes a 39S subunit component of the mitochondial ribosome. Alternative splicing results in multiple transcript variants. Pseudogenes for this gene are found on chromosomes 5 and 12. [provided by RefSeq, May 2014],

Additional antibody information

Specificity MRP-L11 Polyclonal Antibody detects endogenous levels of MRP-L11 protein.
Validation evidence captions Western Blot analysis of various cells using MRP-L11 Polyclonal Antibody diluted at 1:500 | Immunofluorescence analysis of A549 cells, using MRPL11 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using MRPL11 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COS cells, using MRPL11 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: MRP-L11
Collaborator ELK Biotechnology
Partner model ES2827
Target MRP-L11
Research areas>>Ribosome
Reactivity Human,Monkey
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human MRPL11. AA range:21-70
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Duodenum,Lymph,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Monkey
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
MRP-L11
Also known as
MRPL11; CGI-113; 39S ribosomal protein L11; mitochondrial; L11mt; MRP-L11
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Monkey
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPL11. AA range:21-70
Observed band
21kD
Cellular localization
Mitochondrion .
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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