IQGAP1 rabbit pAb from ELK Biotechnology Read more ›
IQGAP1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IQGAP1 in WB, IHC workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
IQ motif containing GTPase activating protein 1(IQGAP1) Homo sapiens This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008],
| Specificity | IQGAP1 Polyclonal Antibody detects endogenous levels of IQGAP1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using IQGAP1 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of HeLa cells using IQGAP1 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of the lysates from K562 cells using IQGAP1 antibody. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES2639 |
| Target | IQGAP1 |
| Research areas | >>Adherens junction, >>Regulation of actin cytoskeleton, >>Proteoglycans in cancer |
| Reactivity | Human,Mouse |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IQGAP1. AA range:247-296 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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