Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97149

eIF2Bγ rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

eIF2Bγ rabbit pAb from ELK Biotechnology Read more ›

eIF2Bγ rabbit pAb
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eIF2Bγ rabbit pAb

Product Description

eIF2Bγ rabbit pAb is a research-use antibody from ELK Biotechnology for studying eIF2Bγ in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009],

Additional antibody information

Specificity eIF2Bγ Polyclonal Antibody detects endogenous levels of eIF2Bγ protein.
Validation evidence captions Western Blot analysis of various cells using eIF2Bγ Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of K562 cells using eIF2Bγ Polyclonal Antibody diluted at 1:1000

Key Facts

Product typePrimary Antibody Target: eIF2Bγ
Collaborator ELK Biotechnology
Partner model ES2243
Target eIF2Bγ
Research areas>>Herpes simplex virus 1 infection
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from eIF2Bγ . at AA range: 240-320
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Blood,Hepatoma,Lymph node,Mammary gland,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
eIF2Bγ
Also known as
EIF2B3; Translation initiation factor eIF-2B subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from eIF2Bγ . at AA range: 240-320
Observed band
50kD
Cellular localization
cytoplasm,cytosol,eukaryotic translation initiation factor 2B complex,
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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