Connexin 25 rabbit pAb from ELK Biotechnology Read more ›
Connexin 25 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Connexin 25 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Connexins, such as GJB7, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008],
| Specificity | Connexin 25 Polyclonal Antibody detects endogenous levels of Connexin 25 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Connexin 25 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from HT-29 cells, using GJB7 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using GJB7 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2027 |
| Target | Connexin 25 |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GJB7. AA range:21-70 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Weakly expressed in placenta. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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