Casein Kinase Iα rabbit pAb from ELK Biotechnology Read more ›
Casein Kinase Iα rabbit pAb is a research-use antibody from ELK Biotechnology for studying Casein Kinase Iα in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the Axin complex.,
| Specificity | Casein Kinase Iα Polyclonal Antibody detects endogenous levels of Casein Kinase Iα protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Casein Kinase Iα Polyclonal Antibody | Immunofluorescence analysis of HeLa cells, using CKI-alpha Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CKI-alpha Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa cells, using CKI-alpha Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1847 |
| Target | Casein Kinase Iα |
| Research areas | >>Wnt signaling pathway, >>Hedgehog signaling pathway, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Human papillomavirus infection, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CKI-alpha. AA range:126-175 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Epithelium,Liver,Lung,Placenta,Skin, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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