Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95766

AGBL4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

AGBL4 rabbit pAb from ELK Biotechnology Read more ›

AGBL4 rabbit pAb
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AGBL4 rabbit pAb

Product Description

AGBL4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AGBL4 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

cofactor:Binds 1 zinc ion per subunit.,function:May play a role in the processing of tubulin.,similarity:Belongs to the peptidase M14 family.,

Additional antibody information

Specificity AGBL4 Polyclonal Antibody detects endogenous levels of AGBL4 protein.
Validation evidence captions Western Blot analysis of various cells using AGBL4 Polyclonal Antibody | Western blot analysis of lysates from A549, HeLa, and Jurkat cells, using AGBL4 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: AGBL4
Collaborator ELK Biotechnology
Partner model ES1610
Target AGBL4
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human AGBL4. AA range:431-480
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Whole embryo,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
AGBL4
Also known as
AGBL4; CCP6; Cytosolic carboxypeptidase 6; ATP/GTP-binding protein-like 4
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human AGBL4. AA range:431-480
Observed band
62kD
Cellular localization
Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Golgi apparatus . Cytoplasm, cytoskeleton, cilium basal body . Colocalizes with gamma-tubulin in the centrioles at interphase and dividing cells and with glutamylated tubulin in basal bodies of ciliated cells. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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