Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94264

TLE1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TLE1 rabbit pAb from ELK Biotechnology Read more ›

TLE1 rabbit pAb
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TLE1 rabbit pAb

Product Description

TLE1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TLE1 in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of TLE1 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: TLE1
Collaborator ELK Biotechnology
Partner model ES12683
Target TLE1
Research areas>>Wnt signaling pathway, >>Notch signaling pathway
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human TLE1 AA range: 300-350
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system In all tissues examined, mostly in brain, liver and muscle.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
TLE1
Also known as
Transducin-like enhancer protein 1 (E(Sp1) homolog) (Enhancer of split groucho-like protein 1) (ESG1)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human TLE1 AA range: 300-350
Observed band
85kD
Cellular localization
Nucleus . Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.337% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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