Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92183

HSP27 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

HSP27 Rabbit pAb from ELK Biotechnology Read more ›

HSP27 Rabbit pAb
Zoom
HSP27 Rabbit pAb

Product Description

HSP27 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying HSP27 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Heat shock protein 27 (Hsp27) also known as heat shock protein beta-1 (HSPB1) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, α-crystallin, Hsp20 and others.Involved in stress resistance and actin organization.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2)C2C12, 3)Mouse Brain, 4) Rat Brain, with HSP27 Rabbit pAb EA155 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using HSP27(EA155)Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: HSP27
Collaborator ELK Biotechnology
Partner model EA155
Target HSP27
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:200-500
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
Why ABMIUM?
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators
Independently handpicked Products selected for quality and fit

Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
HSP27
Also known as
Heat shock protein 27, CMT2F, HSP B1, HSP28, SRP27, Stress responsive protein 27
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
27kDa
Cellular localization
Cytoplasm, Cytoskeleton, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

Customer Reviews

No customer reviews yet.