Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95664

ABHD2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ABHD2 rabbit pAb from ELK Biotechnology Read more ›

ABHD2 rabbit pAb
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ABHD2 rabbit pAb

Product Description

ABHD2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ABHD2 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein containing an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. The function of this protein has not been determined. Alternative splicing of this gene results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity ABHD2 Polyclonal Antibody detects endogenous levels of ABHD2 protein.
Validation evidence captions Western Blot analysis of various cells using ABHD2 Polyclonal Antibody | Immunofluorescence analysis of MCF-7 cells, using ABHD2 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HT29 cells, using ABHD2 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: ABHD2
Collaborator ELK Biotechnology
Partner model ES1572
Target ABHD2
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ABHD2. AA range:251-300
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Present in sperm (at protein level).
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ABHD2
Also known as
ABHD2; LABH2; Abhydrolase domain-containing protein 2; Lung alpha/beta hydrolase 2; Protein PHPS1-2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ABHD2. AA range:251-300
Observed band
48kD
Cellular localization
Cell projection, cilium, flagellum membrane ; Single-pass type II membrane protein . Cell membrane ; Single-pass type II membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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