Collaborator Elabscience
Recombinant ProteinsISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-16771

Recombinant Human MMP-2 Protein

1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpecCatalog # 27076). The specific activity is > 1000 pmoles/min/µg. 2. Immobilized human MMP2 at 10 μg/mL (100 μl/well) can bind human TIMP2/Fc. The EC50 of human TIMP2/Fc is 0.02 μg/mL. (Activation description: The proenzyme needs to be activated by APMA for an activated form)HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human MMP-2 Protein supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Measured by its binding ability in a functional ELISA. Immobilized Human MMP-2 (Cat: PKSH031888) at 2 μg/ml (100 μl/well) can bind Human TIMP2 hFc(Cat: PKSH030472), the EC50 of Human  TIMP2 hFc is 6.0-30.0 ng/mL.
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Measured by its binding ability in a functional ELISA. Immobilized Human MMP-2 (Cat: PKSH031888) at 2 μg/ml (100 μl/well) can bind Human TIMP2 hFc(Cat: PKSH030472), the EC50 of Human  TIMP2 hFc is 6.0-30.0 ng/mL.["1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is > 1, 000 pmoles/min/µg. 2. Immobilized human MMP2 at 10 μg/mL (100 μl/well) can bind human TIMP2/Fc. The EC50 of human TIMP2/Fc is 0.02 μg/mL. (Activation description: The proenzyme needs to be activated by APMA for an activated form) ,72 kDa"]

Product Description

MMP-2 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,MMP-2 ,Recombinant Proteins; predicted molecular weight: 72 kDa.

Quality and formulation: purity: > 90 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: 1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is > 1, 000 pmoles/min/µg. 2. Immobilized human MMP2 at 10 μg/mL (100 μl/well) can bind human TIMP2/Fc. The EC<sub>50</sub> of human TIMP2/Fc is 0.02 μg/mL. (Activation description: The proenzyme needs to be activated by APMA for an activated form). In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 72 kDa.

Database references: UniProt: NP_004521.1 | NCBI Gene: 4313 | Gene symbol: MMP-2.

Research Use Only.

Key Facts

Product typeRecombinant Proteins Target: MMP-2
Collaborator Elabscience
Partner model PKSH031888_100ug
Target MMP-2
Research areasCell biology, Cardiovascular, Cancer, metabolism
Reactivity Human
Purity >90% by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Shelf life 12 months
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryBioactivityBindingCell-BasedIn VivoELISA Capture
Human

Specifications

Target
MMP-2
Also known as
72 kDa Gelatinase, 72 kDa Type IV Collagenase, CLG4, CLG4A, Gelatinase A, MMP-2, MMP-II, MMP2, MONA, Matrix Metalloproteinase-2, TBE-1
Purity
>90% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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