SAP18 rabbit pAb
Anticorps polyclonal anti-SAP18 de lapin de la société ELK Biotechnology Read more ›
Product Description
SAP18 rabbit pAb is an ELK Biotechnology antibody for research workflows involving SAP18.
Background
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2008],
Additional antibody information
| Specificity | This antibody detects endogenous levels of SAP18 at Human/Mouse |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
| Target | SAP18 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000; IHC-p 1:50-300 |
| Species reactivity | Humain ; Souris |
| Cellular localization | Noyau . Cytoplasme . Tache nucléaire . Fait la navette entre le noyau et le cytoplasme (PubMed:16314458). Colocalise avec ACIN1 et SRSF2 dans les taches nucléaires (PubMed:20966198). . |
| Purity | L'anticorps a été purifié par affinité à partir de sérum de lapin par chromatographie d'affinité en utilisant un immunogène spécifique. |
| Form / buffer | Liquide dans du PBS contenant 50 % de glycérol, 0,5 % de BSA et 0,02 % d'azoture de sodium. |
| Research area | >>Transport nucléocytoplasmique ;>>Voie de surveillance des ARNm |
| Host species | Lapin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Non conjugué |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000; IHC-p 1:50-300 |
| Immunogen | Peptide synthétisé dérivé de la SAP18 humaine |
| Purification | L'anticorps a été purifié par affinité à partir de sérum de lapin par chromatographie d'affinité en utilisant un immunogène spécifique. |
Reactivity & Application Validation
2 espèces| Espèce | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Humain | |||||||
| Souris |
| Espèce | Dilution | Notes |
|---|---|---|
| Humain | WB 1:500-2000;IHC-p 1:50-300 | — |
| Souris | — | — |
| Espèce | Dilution | Notes |
|---|
Spécifications
Stockage et stabilité
Conformité et certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.