SARDH lapin pAb de ELK Biotechnology Read more ›
SARDH rabbit pAb is a research-use antibody from ELK Biotechnology for studying SARDH in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2008],
| Specificity | This antibody detects endogenous levels of SARDH at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES13199 |
| Target | SARDA |
| Research areas | >>Glycine, serine and threonine metabolism, >>Metabolic pathways |
| Reactivity | Humain ; Souris ; Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Non conjugué |
| Dilution range | WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Lapin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide synthétisé dérivé de SARDH humain (plage d'AA : 279-329) |
| Concentration | 1 mg/ml |
| Purification | L'anticorps a été purifié par affinité à partir de sérum de lapin par chromatographie d'affinité en utilisant un immunogène spécifique. |
| Assay type | Validation/application des anticorps |
| Expression system | Exprimé dans le pancréas, le foie et le rein. |
| Purity | L'anticorps a été purifié par affinité à partir de sérum de lapin par chromatographie d'affinité en utilisant un immunogène spécifique. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Humain | |||||||
| Souris | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Humain | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Souris | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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