anticorps polyclonal de lapin SCOT1 de chez ELK Biotechnology Read more ›
SCOT1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SCOT1 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008],
| Specificity | This antibody detects endogenous levels of SCOT1 at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES13181 |
| Target | ÉCOS1 |
| Research areas | >>Valine, leucine and isoleucine degradation, >>Butanoate metabolism, >>Metabolic pathways |
| Reactivity | Humain ; Souris ; Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Non conjugué |
| Dilution range | WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Lapin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide synthétisé dérivé de SCOT1 humain, domaine AA : 105-155 |
| Concentration | 1 mg/ml |
| Purification | L'anticorps a été purifié par affinité à partir de sérum de lapin par chromatographie d'affinité en utilisant un immunogène spécifique. |
| Assay type | Validation/application des anticorps |
| Expression system | Abondant dans le cœur, suivi, dans l'ordre, par le cerveau, les reins, les muscles squelettiques et les poumons, alors qu'il est indétectable dans le foie. Exprimé (au niveau des protéines) dans tous les tissus (sauf le foie), le plus abondant dans le myocarde, puis le cerveau, les reins, les glandes surrénales, les muscles squelettiques et les poumons ; également détectable dans les leucocytes et les fibroblastes. |
| Purity | L'anticorps a été purifié par affinité à partir de sérum de lapin par chromatographie d'affinité en utilisant un immunogène spécifique. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Humain | |||||||
| Souris | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Humain | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Souris | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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