PKC ζ (phospho Thr560) rabbit pAb from ELK Biotechnology Read more ›
PKC ζ (phospho Thr560) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PKC ζ in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-PKC ζ (T560) Polyclonal Antibody detects endogenous levels of PKC ζ protein only when phosphorylated at T560. |
|---|---|
| Validation evidence captions | Western Blot analysis of HELA 293T cells using Phospho-PKC ζ (T560) Polyclonal Antibody diluted at 1:2000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC zeta (Phospho-Thr560) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using PKC zeta (Phospho-Thr560) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using PKC zeta (Phospho-Thr560) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6800 |
| Target | PKC ζ |
| Research areas | >>Rap1 signaling pathway, >>Chemokine signaling pathway, >>Sphingolipid signaling pathway, >>Endocytosis, >>Axon guidance, >>Hippo signaling pathway, >>Tight junction, >>Platelet activation, >>Insulin signaling pathway, >>Relaxin signaling pathway, >>Type II diabetes mellitus, >>Insulin resistance, >>AGE-RAGE signaling pathway in diabetic complications, >>Human papillomavirus infection, >>Diabetic cardiomyopathy, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr560. AA range:526-575 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in brain, and to a lesser extent in lung, kidney and testis. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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