PC-PLD1 (phospho Thr147) rabbit pAb from ELK Biotechnology Read more ›
PC-PLD1 (phospho Thr147) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PC-PLD1 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011],
| Specificity | Phospho-PC-PLD1 (T147) Polyclonal Antibody detects endogenous levels of PC-PLD1 protein only when phosphorylated at T147. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES6611 |
| Target | PC-PLD1 |
| Research areas | >>Glycerophospholipid metabolism, >>Ether lipid metabolism, >>Metabolic pathways, >>Ras signaling pathway, >>cAMP signaling pathway, >>Sphingolipid signaling pathway, >>Phospholipase D signaling pathway, >>Endocytosis, >>Fc gamma R-mediated phagocytosis, >>Glutamatergic synapse, >>GnRH signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Pathways in cancer, >>Chemical carcinogenesis - reactive oxygen species, >>Pancreatic cancer, >>Choline metabolism in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human PC-PLD1 (phospho Thr147) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
