Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97240

GPR15 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GPR15 rabbit pAb from ELK Biotechnology Read more ›

GPR15 rabbit pAb
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GPR15 rabbit pAb

Product Description

GPR15 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GPR15 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a G protein-coupled receptor that acts as a chemokine receptor for human immunodeficiency virus type 1 and 2. The encoded protein localizes to the cell membrane. [provided by RefSeq, Nov 2012],

Additional antibody information

Specificity GPR15 Polyclonal Antibody detects endogenous levels of GPR15 protein.
Validation evidence captions Western Blot analysis of various cells using GPR15 Polyclonal Antibody | Immunofluorescence analysis of LOVO cells, using GPR15 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HepG2 cells, using GPR15 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using GPR15 antibody.

Key Facts

Product typePrimary Antibody Target: GPR15
Collaborator ELK Biotechnology
Partner model ES2454
Target GPR15
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GPR15. AA range:201-250
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Liver,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GPR15
Also known as
GPR15; G-protein coupled receptor 15; Brother of Bonzo; BoB
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR15. AA range:201-250
Observed band
40kD
Cellular localization
Cell membrane; Multi-pass membrane protein.
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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