Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94632

S100G Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

S100G Rabbit pAb from ELK Biotechnology Read more ›

S100G Rabbit pAb
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S100G Rabbit pAb

Product Description

S100G Rabbit pAb is a research-use antibody from ELK Biotechnology for studying S100G in IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes calbindin D9K, a vitamin D-dependent calcium-binding protein. This cytosolic protein belongs to a family of calcium-binding proteins that includes calmodulin, parvalbumin, troponin C, and S100 protein. In the intestine, the protein is vitamin D-dependent and its expression correlates with calcium transport activity. The protein may increase Ca2+ absorption by buffering Ca2+ in the cytoplasm and increase ATP-dependent Ca2+ transport in duodenal basolateral membrane vesicles. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous levels of human S100G
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: S100G
Collaborator ELK Biotechnology
Partner model ES13277
Target S100G
Research areas>>Mineral absorption
Reactivity Human,Mouse,Rat
Applications IHC,ELISA
ConjugationUnconjugated
Dilution rangeIHC-p 1:50-200, ELISA(peptide)1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human S100G AA range: 1-80
Concentration 1 mg/ml
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC-p 1:50-200
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
S100G
Also known as
Protein S100-G (Calbindin-D9k;S100 calcium-binding protein G;Vitamin D-dependent calcium-binding protein, intestinal;CABP)
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IHCELISA
Immunogen
Synthesized peptide derived from human S100G AA range: 1-80
Cellular localization
basolateral plasma membrane,apical plasma membrane,
Dilution range
IHC-p 1:50-200, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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