SEC61B Rabbit pAb from ELK Biotechnology Read more ›
SEC61B Rabbit pAb is a research-use antibody from ELK Biotechnology for studying SEC61B in IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq, Jul 2008],
| Specificity | This antibody detects endogenous levels of human SEC61B |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES13170 |
| Target | SEC61B |
| Research areas | >>Protein export, >>Protein processing in endoplasmic reticulum, >>Phagosome, >>Vibrio cholerae infection |
| Reactivity | Human,Mouse,Rat |
| Applications | IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | IHC-p 1:50-200, ELISA(peptide)1:5000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human SEC61B AA range: 30-110 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Embryonic kidney,Epithelium,Stomach mucosa, |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-200 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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