Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM68105

Rat D2D(D-Dimer) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Rat D2D(D-Dimer) ELISA Kit from ELK Biotechnology Read more ›

Rat D2D(D-Dimer) ELISA Kit
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Rat D2D(D-Dimer) ELISA Kit

Product Description

Rat D2D(D-Dimer) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring D2D in Plasma samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat D2D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat D2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat D2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat D2D in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include D 2 Dimer.

Key Facts

Product typeELISA Kits Target: D2D
Collaborator ELK Biotechnology
Partner model ELK4818
Target D2D
Research areasHematology, Cardiovascular biology
Reactivity Rat
Applications ELISA
Assay type Sandwich
Sample types Plasma
Detection range 15.63-1000 ng/mL
Sensitivity 4.8 ng/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
D2D
Also known as
D 2 Dimer
Form / buffer
Pre-coated microplate kit
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
4.8 ng/mL
Detection range
15.63-1000 ng/mL
Sample types
Plasma
Plate format
96-well pre-coated
Species reactivity
Rat

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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