EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring EasyStep Human 25-OH-D in Serum, Plasma samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include 25 Hydroxy Vitamin D;.