Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99260

THIK-2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

THIK-2 rabbit pAb from ELK Biotechnology Read more ›

THIK-2 rabbit pAb
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THIK-2 rabbit pAb

Product Description

THIK-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying THIK-2 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

potassium two pore domain channel subfamily K member 12(KCNK12) Homo sapiens This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity THIK-2 Polyclonal Antibody detects endogenous levels of THIK-2 protein.
Validation evidence captions Western blot analysis of KCNK12 Antibody. The lane on the right is blocked with the KCNK12 peptide. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: THIK-2
Collaborator ELK Biotechnology
Partner model ES6852
Target THIK-2
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human KCNK12. AA range:336-385
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Epithelium,PNS,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
THIK-2
Also known as
KCNK12; Potassium channel subfamily K member 12; Tandem pore domain halothane-inhibited potassium channel 2; THIK-2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHCELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human KCNK12. AA range:336-385
Observed band
47kD
Cellular localization
Membrane ; Multi-pass membrane protein .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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