Mox1 rabbit pAb from ELK Biotechnology Read more ›
Mox1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Mox1 in WB, IHC workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012],
| Specificity | Mox1 Polyclonal Antibody detects endogenous levels of Mox1 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of NOX1 Antibody. The lane on the right is blocked with the NOX1 peptide. | Western blot analysis of the lysates from HeLa cells using NOX1 antibody. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES5532 |
| Target | Mox1 |
| Research areas | >>Osteoclast differentiation, >>AGE-RAGE signaling pathway in diabetic complications, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Chemical carcinogenesis - reactive oxygen species, >>Lipid and atherosclerosis, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Mouse |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NOX1. AA range:436-485 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
