HCCS rabbit pAb from ELK Biotechnology Read more ›
HCCS rabbit pAb is a research-use antibody from ELK Biotechnology for studying HCCS in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
holocytochrome c synthase(HCCS) Homo sapiens The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010],
| Specificity | HCCS Polyclonal Antibody detects endogenous levels of HCCS protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using HCCS Polyclonal Antibody diluted at 1:2000 | Immunofluorescence analysis of MCF7 cells, using Cytochrome c-type Heme Lyase Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using Cytochrome c-type Heme Lyase Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa, HepG2, and COLO cells, using Cytochrome c-type Heme Lyase Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2496 |
| Target | HCCS |
| Research areas | >>Porphyrin metabolism, >>Metabolic pathways |
| Reactivity | Human,Mouse,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cytochrome c-type Heme Lyase. AA range:81-130 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Liver,Ovary, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Monkey | — | — |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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