Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97141

ECP rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ECP rabbit pAb from ELK Biotechnology Read more ›

ECP rabbit pAb
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ECP rabbit pAb

Product Description

ECP rabbit pAb is a research-use antibody from ELK Biotechnology for studying ECP in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014],

Additional antibody information

Specificity ECP Polyclonal Antibody detects endogenous levels of ECP protein.
Validation evidence captions Western Blot analysis of various cells using ECP Polyclonal Antibody | Western blot analysis of lysate from 293 cells, usiung ECP antibody.

Key Facts

Product typePrimary Antibody Target: ECP
Collaborator ELK Biotechnology
Partner model ES2221
Target ECP
Research areas>>Asthma
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ECP. AA range:102-151
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Colon,Fetal liver,Peripheral blood granulocyte,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ECP
Also known as
RNASE3; ECP; RNS3; Eosinophil cationic protein; ECP; Ribonuclease 3; RNase 3
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ECP. AA range:102-151
Observed band
18kD
Cellular localization
Secreted. Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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