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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95247

MRI rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MRI rabbit pAb from ELK Biotechnology Read more ›

MRI rabbit pAb
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MRI rabbit pAb

Product Description

MRI rabbit pAb is a research-use antibody from ELK Biotechnology for studying MRI in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of MRI at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody› Target: MRI
Collaborator ELK Biotechnology
Partner model ES14710
Target MRI
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human MRI AA range: 40-90
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 —
Mouse — —
Rat — —
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
MRI
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHCELISA
Immunogen
Synthesized peptide derived from human MRI AA range: 40-90
Cellular localization
Isoform 1: Cytoplasm . Nucleus . Chromosome . Nuclear localization may depend upon interaction with XRCC5/Ku80 and XRCC6/Ku70 heterodimer (PubMed:24610814). Localizes to DNA damage sites (PubMed:27063109). .; Isoform 3: Cytoplasm . Some nuclear localization may be due to passive diffusion. .; Isoform 4: Cytoplasm . Nucleus . Nuclear localization may depend upon interaction with XRCC5/Ku80 and XRCC6/Ku70 heterodimer and increases upon etoposide treatment. .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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