GSK3β (phospho Ser9) rabbit pAb from ELK Biotechnology Read more ›
GSK3β (phospho Ser9) rabbit pAb is a research-use antibody from ELK Biotechnology for studying GSK3β in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],
| Specificity | Phospho-GSK3β (S9) Polyclonal Antibody detects endogenous levels of GSK3β protein only when phosphorylated at S9. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:500 | Western Blot analysis of RAT-kidney cells using Phospho-GSK3β (S9) Polyclonal Antibody diluted at 1:500 | Western blot analysis of GSK3β (Phospho-Ser9) Antibody. The lane on the right is blocked with the GSK3β (Phospho-Ser9) peptide. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES1420 |
| Target | GSK3β |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>ErbB signaling pathway, >>Chemokine signaling pathway, >>Cell cycle, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>Wnt signaling pathway, >>Hedgehog signaling pathway, >>Axon guidance, >>Hippo signaling pathway, >>Focal adhesion, >>Signaling pathways regulating pluripotency of stem cells, >>IL-17 signaling pathway, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>Neurotrophin signaling pathway, >>Dopaminergic synapse, >>Insulin signaling pathway, >>Melanogenesis, >>Prolactin signaling pathway, >>Thyroid hormone signaling pathway, >>Insulin resistance, >>Non-alcoholic fatty liver disease, >>Cushing syndrome, >>Growth hormone synthesis, secretion and action, >>Alcoholic liver disease, >>Alzheimer disease, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Shigellosis, >>Yersinia infection, >>Hepatitis C, >>Measles, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpes |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human GSK3β (phospho Ser9) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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