Jun B (phospho Ser259) rabbit pAb from ELK Biotechnology Read more ›
Jun B (phospho Ser259) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Jun B in WB, IHC, IF, ELISA, IP workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.,induction:By growth factors.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Jun subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as an homodimer or as an heterodimer with another member of the jun/fos family.,
| Specificity | Phospho-Jun B (S259) Polyclonal Antibody detects endogenous levels of Jun B protein only when phosphorylated at S259. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Jun B (S259) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunB (Phospho-Ser259) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa cells, using JunB (Phospho-Ser259) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1351 |
| Target | Jun B |
| Research areas | >>Osteoclast differentiation, >>TNF signaling pathway, >>Growth hormone synthesis, secretion and action |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA,IP |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human JunB around the phosphorylation site of Ser259. AA range:226-275 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Adipose tissue,Epithelium,Pancreas,Placenta,Prostat |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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