Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92407

CD20 Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

CD20 Mouse mAb from ELK Biotechnology Read more ›

CD20 Mouse mAb
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CD20 Mouse mAb

Product Description

CD20 Mouse mAb is a research-use antibody from ELK Biotechnology for studying CD20 in IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca2+ channel subunit.

Additional antibody information

Validation evidence captions IHC staining of Human tonsil tissue paraffin-embedded with CD20 mouse mAb(2F4)diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: CD20
Collaborator ELK Biotechnology
Partner model EM1048
Target CD20
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications IHC
ConjugationUnconjugated
Dilution rangeIHC 1:200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC 1:200
Rat
Mouse

Specifications

Target
CD20
Also known as
B lymphocyte antigen CD20, B1, Bp35, LEU 16, MS4A1, MS4A1,MS4A2, S7
Concentration
1mg/mL
Working concentration
IHC 1:200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IHC
Immunogen
Synthetic Peptide
Observed band
35-37kDa
Cellular localization
Membrane
Dilution range
IHC 1:200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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