Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99784

CD56 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CD56 rabbit pAb from ELK Biotechnology Read more ›

CD56 rabbit pAb
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CD56 rabbit pAb

Product Description

CD56 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD56 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011],

Additional antibody information

Specificity CD56 Polyclonal Antibody detects endogenous levels of CD56 protein.
Validation evidence captions Western blot analysis of mouse-lung mouse-heart lysis using NCAM1 antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200 | Western Blot analysis of Hela cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: CD56
Collaborator ELK Biotechnology
Partner model ES8423
Target CD56
Research areas>>Cell adhesion molecules, >>Prion disease
Reactivity Human,Rat,Mouse
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human NCAM1. AA range:250-300
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Brain cortex,Kidney,Ovary,Plasma,Skeletal muscle,Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CD56
Also known as
NCAM1; NCAM; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD56
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human NCAM1. AA range:250-300
Observed band
94kD
Cellular localization
Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 2: Cell membrane; Single-pass type I membrane protein.; Isoform 3: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform 4: Cell membrane ; Lipid-anchor, GPI-anchor .; Isoform 5: Secreted.; Isoform 6: Secreted .
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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