Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98671

Nup160 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Nup160 rabbit pAb from ELK Biotechnology Read more ›

Nup160 rabbit pAb
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Nup160 rabbit pAb

Product Description

Nup160 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Nup160 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

nucleoporin 160(NUP160) Homo sapiens NUP160 is 1 of up to 60 proteins that make up the 120-MD nuclear pore complex, which mediates nucleoplasmic transport.[supplied by OMIM, Apr 2004],

Additional antibody information

Specificity Nup160 Polyclonal Antibody detects endogenous levels of Nup160 protein.
Validation evidence captions Western Blot analysis of Hela cells using Nup160 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western blot analysis of lysates from NIH/3T3, A549, and HepG2 cells, using NUP160 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: Nup160
Collaborator ELK Biotechnology
Partner model ES5327
Target Nup160
Research areas>>Nucleocytoplasmic transport, >>Amyotrophic lateral sclerosis
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human NUP160. AA range:392-441
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Bone marrow,Epithelium,Muscle,Skin,Small intestine,Testis,Trachea,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Nup160
Also known as
NUP160; KIAA0197; NUP120; Nuclear pore complex protein Nup160; 160 kDa nucleoporin; Nucleoporin Nup160
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human NUP160. AA range:392-441
Observed band
140kD
Cellular localization
Nucleus, nuclear pore complex .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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