Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98580

Emx1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Emx1 rabbit pAb from ELK Biotechnology Read more ›

Emx1 rabbit pAb
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Emx1 rabbit pAb

Product Description

Emx1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Emx1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.,similarity:Belongs to the EMX homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,tissue specificity:Cerebral cortex.,

Additional antibody information

Specificity Emx1 Polyclonal Antibody detects endogenous levels of Emx1 protein.
Validation evidence captions Western Blot analysis of HuvEC cells using Emx1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western blot analysis of the lysates from Jurkat cells using EMX1 antibody.

Key Facts

Product typePrimary Antibody Target: Emx1
Collaborator ELK Biotechnology
Partner model ES5114
Target Emx1
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human EMX1. AA range:181-230
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Cerebral cortex.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Emx1
Also known as
EMX1; Homeobox protein EMX1; Empty spiracles homolog 1; Empty spiracles-like protein 1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human EMX1. AA range:181-230
Observed band
28kD
Cellular localization
Nucleus . Cytoplasm . Might be shuttling between the nucleus and the cytoplasm.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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