Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97169

Epsin 1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Epsin 1 rabbit pAb from ELK Biotechnology Read more ›

Epsin 1 rabbit pAb
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Epsin 1 rabbit pAb

Product Description

Epsin 1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Epsin 1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the epsin protein family. The encoded protein binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Loss of function of this gene is associated with reduced tumor growth and progression in certain cancer types. [provided by RefSeq, Mar 2016],

Additional antibody information

Specificity Epsin 1 Polyclonal Antibody detects endogenous levels of Epsin 1 protein.
Validation evidence captions Western Blot analysis of various cells using Epsin 1 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of MOuse-brain cells using Epsin 1 Polyclonal Antibody diluted at 1:1000

Key Facts

Product typePrimary Antibody Target: Epsin 1
Collaborator ELK Biotechnology
Partner model ES2284
Target Epsin 1
Research areas>>Endocytosis
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from Epsin 1 . at AA range: 140-220
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Epithelium,Mammary gland,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Epsin 1
Also known as
EPN1; Epsin-1; EH domain-binding mitotic phosphoprotein; EPS-15-interacting protein 1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from Epsin 1 . at AA range: 140-220
Observed band
85kD
Cellular localization
Cytoplasm . Cell membrane ; Peripheral membrane protein . Nucleus . Membrane, clathrin-coated pit . Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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