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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97039

GAP43 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GAP43 rabbit pAb from ELK Biotechnology Read more ›

GAP43 rabbit pAb
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GAP43 rabbit pAb

Product Description

GAP43 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GAP-43 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity The antibody detects endogenous GAP43 protein.
Validation evidence captions Western blot analysis of 1) Hela, 2) Mouse Brain, 3) Rat Brain tissue, diluted at 1:3000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody› Target: GAP-43
Collaborator ELK Biotechnology
Partner model ES20741
Target GAP-43
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB: 1:1000-3000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein of Neuromodulin
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Alzheimer cortex,Brain,Subthalamic nucleus,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB: 1:1000-3000 —
Mouse — —
Rat — —

Specifications

Target
GAP-43
Also known as
GAP43; Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46
Working concentration
WB: 1:1000-3000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Recombinant Protein of Neuromodulin
Observed band
38,43kD
Cellular localization
Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, growth cone membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, synapse . Cell projection, filopodium membrane ; Peripheral membrane protein . Perikaryon . Cell projection, dendrite . Cell projection, axon . Cytoplasm . Cytoplasmic surface of growth cone and synaptic plasma membranes. .
Dilution range
WB: 1:1000-3000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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