Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92594

HP-1α (5E3) Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

HP-1α (5E3) Mouse mAb from ELK Biotechnology Read more ›

HP-1α (5E3) Mouse mAb
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HP-1α (5E3) Mouse mAb

Product Description

HP-1α (5E3) Mouse mAb is a research-use antibody from ELK Biotechnology for studying HP-1α (5E3) in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin.

Additional antibody information

Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1α(EM1366)Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1α (EM1366)Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1 α (EM1366)Mouse mAb diluted at 1:200 | Western blot analysis of 1) Hela Cell Lysate, 2)3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1α (EM1366)Mouse mAb diluted at 1:1000.

Key Facts

Product typePrimary Antibody Target: HP-1α (5E3)
Collaborator ELK Biotechnology
Partner model EM1366
Target HP-1α (5E3)
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-1,000 IHC 1:100-200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-1
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
HP-1α (5E3)
Also known as
Antigen p25, CBX5, HEL25, Heterochromatin protein 1
Concentration
1mg/mL
Working concentration
WB 1:500-1,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
22kDa
Cellular localization
Cytoplasm, Membrane, Nucleus
Dilution range
WB 1:500-1,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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