Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92350

ATG5 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ATG5 Rabbit pAb from ELK Biotechnology Read more ›

ATG5 Rabbit pAb 50µL/100µL
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ATG5 Rabbit pAb 50µL/100µL

Product Description

ATG5 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying ATG5 in IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Additional antibody information

Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human Hepatocarcinoma Tissue using ATG5 (EA350)Rabbit pAb diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 (EA350)Rabbit pAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: ATG5
Collaborator ELK Biotechnology
Partner model EA350
Target ATG5
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications IHC
ConjugationUnconjugated
Dilution rangeIHC 1:100-200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC 1:100-200
Mouse
Rat

Specifications

Target
ATG5
Also known as
APG5, APG5L,ASP,Autophage Cysteine protein5 antibody
Concentration
1mg/mL
Working concentration
IHC 1:100-200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IHC
Immunogen
Recombinant Protein
Observed band
32kDa
Cellular localization
Cytoplasm, Membrane
Dilution range
IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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