TRH-R1 rabbit pAb from ELK Biotechnology Read more ›
TRH-R1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TRH-R1 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a G protein-coupled receptor for thyrotropin-releasing hormone (TRH). Upon binding to TRH, this receptor activates the inositol phospholipid-calcium-protein kinase C transduction pathway. Mutations in this gene have been associated with generalized thyrotropin-releasing hormone resistance. [provided by RefSeq, Sep 2011],
| Specificity | TRH-R1 Polyclonal Antibody detects endogenous levels of TRH-R1 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using TRHR Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COS7 cells, using TRHR Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using TRHR antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7446 |
| Target | TRH-R1 |
| Research areas | >>Calcium signaling pathway, >>Neuroactive ligand-receptor interaction |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TRHR. AA range:195-244 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Pituitary,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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