IRF-3 (phospho Ser386) rabbit pAb from ELK Biotechnology Read more ›
IRF-3 (phospho Ser386) rabbit pAb is a research-use antibody from ELK Biotechnology for studying IRF-3 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],
| Specificity | Phospho-IRF-3 (S386) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S386. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using IRF-3 (Phospho-Ser386) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES5922 |
| Target | IRF-3 |
| Research areas | >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>Cytosolic DNA-sensing pathway, >>Alcoholic liver disease, >>Shigellosis, >>Pertussis, >>Yersinia infection, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Human cytomegalovirus infection, >>Influenza A, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>Epstein-Barr virus infection, >>Human immunodeficiency virus 1 infection, >>Coronavirus disease - COVID-19, >>Viral carcinogenesis, >>Lipid and atherosclerosis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser386. AA range:352-401 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed constitutively in a variety of tissues. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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