DGK-η rabbit pAb from ELK Biotechnology Read more ›
DGK-η rabbit pAb is a research-use antibody from ELK Biotechnology for studying DGK-η in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
diacylglycerol kinase eta(DGKH) Homo sapiens This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014],
| Specificity | DGK-η Polyclonal Antibody detects endogenous levels of DGK-η protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HeLa cells, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using DGKH Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4959 |
| Target | DGK-η |
| Research areas | >>Glycerolipid metabolism, >>Glycerophospholipid metabolism, >>Metabolic pathways, >>Phosphatidylinositol signaling system, >>Phospholipase D signaling pathway, >>Choline metabolism in cancer |
| Reactivity | Human,Mouse |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DGKH. AA range:771-820 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 1: Expressed only in testis, kidney and colon. ; Isoform 2: Ubiquitously expressed. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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