CD203c rabbit pAb from ELK Biotechnology Read more ›
CD203c rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD203c in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Oct 2015],
| Specificity | CD203c Polyclonal Antibody detects endogenous levels of CD203c protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of K562, MCF7 cells using CD203c Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of rat-kidney hela 293T SH-SY5Y MOUSE-KIDNEY MOUSE-SPLEEN MOUSE-LUNG using ENPP3 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of mouse-kidney lysis using CD203c antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-prostate-cancer, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES4004 |
| Target | CD203c |
| Research areas | >>Purine metabolism, >>Pyrimidine metabolism, >>Starch and sucrose metabolism, >>Riboflavin metabolism, >>Nicotinate and nicotinamide metabolism, >>Pantothenate and CoA biosynthesis, >>Metabolic pathways, >>Nucleotide metabolism |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human ENPP3. AA range:281-330 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Detected on bile ducts in liver, and in blood serum (at protein level) (PubMed:15072822). Detected in prostate and uterus (PubMed:9344668). Detected on basophils, but not neutrophils (PubMed:11342463). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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