MARK2 rabbit pAb from ELK Biotechnology Read more ›
MARK2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MARK2 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
microtubule affinity regulating kinase 2(MARK2) Homo sapiens This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009],
| Specificity | MARK2 Polyclonal Antibody detects endogenous levels of MARK2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using MARK2 Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using MARK2 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COS7 cells, using MARK2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from 293 cells using MARK2 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2747 |
| Target | MARK2 |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MARK2. AA range:10-59 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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