Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97252

GRIN1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GRIN1 rabbit pAb from ELK Biotechnology Read more ›

GRIN1 rabbit pAb
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GRIN1 rabbit pAb

Product Description

GRIN1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GRIN1 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:May be involved in neurite outgrowth.,PTM:Palmitoylation on Cys-999 and/or Cys-1000 is required for membrane targeting.,subcellular location:Highly enriched in growth cone.,subunit:Interacts with activated forms of GNAI1, GNAO1 and GNAZ.,tissue specificity:Widely expressed in the central nervous system, with highest levels in spinal cord.,

Additional antibody information

Specificity GRIN1 Polyclonal Antibody detects endogenous levels of GRIN1 protein.
Validation evidence captions Western Blot analysis of various cells using GRIN1 Polyclonal Antibody diluted at 1:500 | Western Blot analysis of HT29 cells using GRIN1 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from HT-29 cells, using GPRIN1 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: GRIN1
Collaborator ELK Biotechnology
Partner model ES2474
Target GRIN1
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GPRIN1. AA range:231-280
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed in the central nervous system, with highest levels in spinal cord.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GRIN1
Also known as
GPRIN1; KIAA1893; G protein-regulated inducer of neurite outgrowth 1; GRIN1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GPRIN1. AA range:231-280
Observed band
102kD
Cellular localization
Cell membrane ; Lipid-anchor . Cell projection, growth cone . Highly enriched in growth cone. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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