Flag-Tag(HRP Conjugated) Mouse mAb from ELK Biotechnology Read more ›
Flag-Tag(HRP Conjugated) Mouse mAb is a research-use antibody from ELK Biotechnology for studying Flag-Tag(HRP Conjugated) in WB workflows with listed reactivity for Epitope tag. Specifications and supporting documents below should be reviewed when planning the experiment.
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
| Validation evidence captions | Western blot analysis of Recombinant protein with EM1024 diluted at 1) 1:5,000 2) 1:10,000 |
|---|
| Collaborator | ELK Biotechnology |
| Partner model | EM1024 |
| Target | Flag-Tag(HRP Conjugated) |
| Research areas | Antibody Research |
| Reactivity | Epitope tag |
| Applications | WB |
| Conjugation | HRP |
| Dilution range | WB 1:5,000-10,000 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Species | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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