A-Raf (phospho Tyr302) rabbit pAb from ELK Biotechnology Read more ›
A-Raf (phospho Tyr302) rabbit pAb is a research-use antibody from ELK Biotechnology for studying A-Raf in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012],
| Specificity | Phospho-A-Raf (Y302) Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at Y302. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using A-RAF (Phospho-Tyr302) Antibody | Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5953 |
| Target | A-Raf |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>MAPK signaling pathway, >>ErbB signaling pathway, >>FoxO signaling pathway, >>Vascular smooth muscle contraction, >>Natural killer cell mediated cytotoxicity, >>Long-term potentiation, >>Serotonergic synapse, >>Long-term depression, >>Regulation of actin cytoskeleton, >>Insulin signaling pathway, >>Progesterone-mediated oocyte maturation, >>Parathyroid hormone synthesis, secretion and action, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Alcoholism, >>Hepatitis C, >>Hepatitis B, >>Pathways in cancer, >>Proteoglycans in cancer, >>Chemical carcinogenesis - reactive oxygen species, >>Colorectal cancer, >>Renal cell carcinoma, >>Pancreatic cancer, >>Endometrial cancer, >>Glioma, >>Prostate cancer, >>Melanoma, >>Bladder cancer, >>Chronic myeloid leukemia, >>Acute myeloid leukemia, >>Non-small cell lung cancer, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302. AA range:276-325 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Predominantly in urogenital tissues. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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