Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98494

ABCA13 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ABCA13 rabbit pAb from ELK Biotechnology Read more ›

ABCA13 rabbit pAb
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ABCA13 rabbit pAb

Product Description

ABCA13 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ABCA13 in IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

In human, the ATP-binding cassette (ABC) family of transmembrane transporters has at least 48 genes and 7 gene subfamilies. This gene is a member of ABC gene subfamily A (ABCA). Genes within the ABCA family typically encode several thousand amino acids. Like other ABC transmembrane transporter proteins, this protein has 12 or more transmembrane alpha-helix domains that likely arrange to form a single central chamber with multiple substrate binding sites. It is also predicted to have two large extracellular domains and two nucleotide binding domains as is typical for ABCA proteins. Alternative splice variants have been described but their biological validity has not been demonstrated.[provided by RefSeq, Mar 2009],

Additional antibody information

Specificity ABCA13 Polyclonal Antibody detects endogenous levels of ABCA13 protein.
Validation evidence captions Immunofluorescence analysis of A549 cells, using ABCA13 Antibody. The picture on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: ABCA13
Collaborator ELK Biotechnology
Partner model ES4922
Target ABCA13
Research areas>>ABC transporters
Reactivity Human,Rat,Mouse
Applications IF,ELISA
ConjugationUnconjugated
Dilution rangeImmunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ABCA13. AA range:2251-2300
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Significantly expressed in the bone marrow, trachea, testis, thyroid and lung as well as in skin fibroblasts.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
Human Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ABCA13
Also known as
ABCA13; ATP-binding cassette sub-family A member 13
Concentration
1 mg/ml
Working concentration
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ABCA13. AA range:2251-2300
Cellular localization
Cytoplasmic vesicle membrane ; Multi-pass membrane protein .
Dilution range
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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