Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98366

Ataxin-2L rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Ataxin-2L rabbit pAb from ELK Biotechnology Read more ›

Ataxin-2L rabbit pAb
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Ataxin-2L rabbit pAb

Product Description

Ataxin-2L rabbit pAb is a research-use antibody from ELK Biotechnology for studying Ataxin-2L in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes an ataxin type 2 related protein of unknown function. This protein is a member of the spinocerebellar ataxia (SCAs) family, which is associated with a complex group of neurodegenerative disorders. Several alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Ataxin-2L Polyclonal Antibody detects endogenous levels of Ataxin-2L protein.
Validation evidence captions Western Blot analysis of 293T cells using Ataxin-2L Polyclonal Antibody diluted at 1:2000 | Western blot analysis of lysates from NIH/3T3 and RAW264.7 cells, using ATXN2L Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from 293 cells using ATXN2L antibody.

Key Facts

Product typePrimary Antibody Target: Ataxin-2L
Collaborator ELK Biotechnology
Partner model ES4628
Target Ataxin-2L
Research areas>>Amyotrophic lateral sclerosis, >>Spinocerebellar ataxia, >>Pathways of neurodegeneration - multiple diseases
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ATXN2L. AA range:571-620
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed at high levels in thymus, lymph node, spleen, fetal kidney and adult testis. Constitutively associated with MPL and EPOR in hematopoietic cells.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Ataxin-2L
Also known as
ATXN2L; A2D; A2LG; A2LP; A2RP; Ataxin-2-like protein; Ataxin-2 domain protein; Ataxin-2-related protein
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ATXN2L. AA range:571-620
Observed band
113kD
Cellular localization
Membrane ; Peripheral membrane protein . Cytoplasm . Nucleus speckle . Cytoplasmic granule . Predominantly cytoplasmic but is also detected in nuclear speckles (PubMed:23209657). Component of cytoplasmic stress granules (PubMed:23209657). Inhibition of methylation alters nuclear localization (PubMed:25748791). Methylation does not seem to be required for localization to stress granules under stress conditions (PubMed:25748791). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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