IL-10 rabbit pAb from ELK Biotechnology Read more ›
IL-10 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IL-10 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011],
| Specificity | IL-10 Polyclonal Antibody detects endogenous levels of IL-10 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HepG2, Mouse liver cells using IL-10 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded Human Cerebral cortex. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES3868 |
| Target | IL-10 |
| Research areas | >>Cytokine-cytokine receptor interaction, >>Viral protein interaction with cytokine and cytokine receptor, >>FoxO signaling pathway, >>C-type lectin receptor signaling pathway, >>JAK-STAT signaling pathway, >>T cell receptor signaling pathway, >>Intestinal immune network for IgA production, >>Pertussis, >>Yersinia infection, >>Leishmaniasis, >>Chagas disease, >>African trypanosomiasis, >>Malaria, >>Toxoplasmosis, >>Amoebiasis, >>Staphylococcus aureus infection, >>Tuberculosis, >>Asthma, >>Autoimmune thyroid disease, >>Inflammatory bowel disease, >>Systemic lupus erythematosus, >>Allograft rejection |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human IL10. AA range:71-120 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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