Vasohibin rabbit pAb from ELK Biotechnology Read more ›
Vasohibin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Vasohibin in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
caution:Although probably secreted, it lacks a canonical signal sequence.,function:Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.,induction:By VEGF.,PTM:2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.,similarity:Belongs to the vasohibin family.,tissue specificity:Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.,
| Specificity | Vasohibin Polyclonal Antibody detects endogenous levels of Vasohibin protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Vasohibin Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of VASH1 Antibody. The lane on the right is blocked with the VASH1 peptide. | Immunohistochemical analysis of paraffin-embedded human colon. 1,primary Antibody was diluted at 1:200(4° overnight). 2, EDTA pH 9.0 was used for antigen retrieval. 3 Reday to use Secondary antibody( catalog: RS0011) was used at 37°C, 30min | Immunohistochemical analysis of paraffin-embedded human brain. 1,primary Antibody was diluted at 1:200(4° overnight). 2, EDTA pH 9.0 was used for antigen retrieval 3 Reday to use Secondary antibody( catalog: RS0011) was used at 37°C, 30min |
| Collaborator | ELK Biotechnology |
| Partner model | ES3685 |
| Target | Vasohibin |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human VASH1. AA range:261-310 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Preferentially expressed in endothelial cells (PubMed:15467828, PubMed:16707096). Highly expressed in fetal organs (PubMed:15467828). Expressed in brain and placenta, and at lower level in heart and kidney (PubMed:15467828). Highly detected in microvessels endothelial cells of atherosclerotic lesions (PubMed:16707096). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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